Hello, Here is a brief summary of the answers I received concerning the antibody conjugation methods. Several people that answered used the Molecular Probes Alexa kits for 100µg of antibody with success. The Qdots labelling seems to work as well. Concerning the Zenon conjugation that allows the staining of the exact quantity of antibody needed for one tube/experiment, I received divergent experiences. This staining seems to be simple and rapid however one mentioned that "the complexes dissociate rather rapidly (for some antibodies) and have to be used within 30-60 minutes of forming the complexes for reasonable performance". Notably, it seems that the conjugation is dependant of the antibody heavy chain type, thus you have to choose carefully your kit depending on your antibody. In conclusion, it seems to work, is expensive for a systematic use but may be useful to test new antibodies/fluorochrome combinations. Another product from Innova Biosciences has been mentioned twice as a good alternative, notably the conjugation seems stable up to one year. Nobody had experience with the kit form AbD Serotec. Finally we choose to get one Alexa microscale kit from MP and one Zenon kit to try and to use with exotic antibodies. Thanks to all for your help! Benoit -- Benoît Labarthe, PharmD Institut de Cardiologie de Montréal Montreal Heart Institute Centre de Recherche, Laboratoire du Dr.Théroux 5000 Bélanger Est Montréal Québec Canada H1T 1C8 Tel (514)-376 3330 ext 3017 Fax (514)- 376 1076Received on Sat Mar 8 15:18:00 2008
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