RE: Looking for subG1

""gold-standard" cell line know for exhibiting a sub-G1 population under
a certain treatment protocol"

IL-3 or IL-7 dependent cell lines, under cytokine withdrawal
293 cells treated with TNF-alpha
fresh mouse thymocytes, treated with corticosteroid

for subG1, be sure you are not excluding them in your FSC threshold setting

=======================================================
Rachel M. Gerstein, Ph.D.
Associate Professor
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX) 



-----Original Message-----
From: Michelle Mok Meng Huang [mailto:michellemok@bsf.a-star.edu.sg]
Sent: Mon 2/18/2008 9:24 PM
To: cyto-inbox
Subject: Looking for subG1
 
Dear flowers,

 

I have a customer here performing analysis on my LSR II looking for a
sub-G1 population. Trouble is, we haven't seen any. So we're not sure if
his treatment protocol has an issue (still in optimization) or that the
cells really do not have a sub-G1 population. So is there is a
"gold-standard" cell line know for exhibiting a sub-G1 population under
a certain treatment protocol? 

 

Any help would be most appreciated.

 

Thank you,

 

Michelle Mok

Research Officer

Flow Cytometry