Absolute Quantification of Intracellular Cytokines

Hello All,

I have been scanning references in pubmed for an answer to my subject line.
It appears that the accepted way to report intracellular cytokines is via
MFI or number of positive cells. I was curious of anyone is aware of a
³standard² that you could run, which would enable you to provide an absolute
quantification (as a pg/ml for instance). We typically run stimulations and
measure extracellular cytokine production with ELISA and it would be useful
to relate intracellular production back in the same units. I do not know if
this is even possible, but I suspect that it may be. For instance I know
that the multiplex bead arrays for flow involve a standard curve. If anyone
has any tips or suggestions, please let me know. Thanks in advance,

Brian
-- 
Brian K. McFarlin, PhD
Assistant Professor Exercise Physiology / Nutrition
University of Houston
Department of Health and Human Performance
3855 Holman St.
Houston, Texas 77204-6015
(713) 743-9929 phone
(713) 743-9860 fax