sticky LMH cells

From: Andrea Bree <ABree@wyeth.com>
Date: Fri Feb 08 2008 - 14:41:11 EST

We are trying to FACS analyse LMH cells (a chicken hepatoma, epithelial cell line).  They are extremely clumpy.  We have tried trypsin, EDTA, EGTA, trypsin-EDTA ,Ca/Mg free buffer, passing the cells through a prefilter, but the cells still clump terribly. Any suggestions?
Andrea Bree
Wyeth Research

Received on Mon Feb 11 14:38:00 2008

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