Our Aria does not have contamination problems. This could be just luck. But our routine is as follows: We NEVER run anything through the sheath lines except autoclaved PBS. We have purchased extra sheath tanks. We fill them with sheath fluid and autoclave them. We switch tanks as neatly as possible when a tank runs out. And that's it. My theory is that there should not be any contamination in the sheath lines if the only thing that has passed through those lines is sterile PBS. Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, NH 03756 USA tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu www.dartmouth.edu/~celllabReceived on Thu Jan 31 13:58:00 2008
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