Re: [ TdT Antibody Control? ]

Hi anonymous from somewhere on planet earth,
If you have access to an amnis machine you could get an answer without a 
control Ab (although a biological control would still be needed, of course), 
and it'd be quite elegant I think. I don't know if the resolution is enough 
to resolve real TdT staining patterns from "unspecific" nuclear staining 
though. Also you can do controls that exclude one step of the preparation to 
see for unspecific staining of the reagent(s). Finally, if still unsure, you 
can take your cells after preparing them and look at them at the microscope.

Best regards,

Uriel.
Uriel Trahtemberg, M.Sc.
MD/PhD student
The Laboratory for Cellular and Molecular Immunology
The Hebrew University - Hadassah Medical Organization
Jerusalem - ISRAEL


----- Original Message ----- 
From: <slsacia@gundluth.org>
To: cyto-inbox
Sent: Friday, December 28, 2007 20:33
Subject: [ TdT Antibody Control? ]


>
>
> Does anyone have a recommendation for what to use for TdT  antibody
> Control.
> 


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