Hi, the staining for intracellular cytokines includes a 4 hour incubation at 37C with brefeldin A, PMA, and ionophore. Does this activation change the level of FoxP3 detected by intracellular staining ? Kind regards and Merry Christmas Ruedi ---------------------------------------------------- Rudolf K Braun, Ph.D. Assistant Professor Thoracic CV Surgery Loyola University Medical Center Bldg: 101; Rm: 2711 2160 South First Avenue Maywood, IL 60153Received on Fri Dec 21 11:38:00 2007
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