Dear Flowers, Am setting up a panel on a 6-color FACScanto to characterize stem cells in peripheral blood, and running into the usual planning problem on what fluorochromes to assign to particular antibodies. The main hassle is caused by the fact that CD133 (miltenyi) and KDR (R&D) are only available in PE and APC, so have to work around these. To make a long story short, there are two options I seem to be left with: 1) Substitute CD34 PE (clone 8G12, BD) for PerCP. Arseniev (1999) concluded PerCP was not really bad, but not really great either. What is your experience?? Was unable find any systematic comparison in the literature that looked at PE-Cy7 or APC-Cy7. Does anyone have good validation data? 2) Alternatively, I can buy CD133 biotin and label it with FITC. Does anyone have good experience with that approach? What can I expect with regard to signal strength, background, and prep hassle? Your advise is very much appreciated! Kind wishes, Jos Jos A. Bosch, PhD Lecturer in Exercise and Behavioural Immunology School of Sport and Exercise Sciences University of Birmingham Birmingham B15 2TT United Kingdom T: (+44) 0121 4148105 F: (+44) 0121 4144121 http://www.sportex.bham.ac.uk/staff/boschjaReceived on Thu Dec 20 13:18:00 2007
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