Whole Blood preps

From: Eric P Miller <Eric.Miller@ed.ac.uk>
Date: Mon Dec 17 2007 - 13:42:07 EST
Hi folks, sorry about the blank posting: my hands are not so steady  
with all the excitement of Christmas coming!
I'm posting to canvass opinion on whether the use of FCS in lyse-no  
wash preparations of whole blood is regarded as helpful or not. We  
don't use it in our panels, four colours on a Coulter XL, not always  
human samples, but I was given a paper by my manager from a different  
group doing similar work who do use it. I've used it in the past on  
indirect techniques, but always washed it out before running samples.  
Obviously this is not possible in a lyse-no wash setup.
Anybody out there have strong opinions? Thanks, and seasons greetings!

-- 
Eric Miller


"Never ascribe to malice what can be put down to incompetence"
				      -Robert A Heinlein


-- 
The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.
Received on Tue Dec 18 12:38:00 2007

This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST