Date: Fri Dec 14 2007 - 12:40:14 EST
PHA causes severe cells agglutination and it is difficult if not impossible to break up the aggregates. PHA M for has stronger agglutinating properties than PHA P, but the latter also agglutinates the cells. The aggregates tend to disperse after 48 h of incubation. Pokeweed Mitogen (Phytolacca) is another polyvalent mitogen - it does not induce agglutination but it activates B cells. Zbigniew Darzynkiewicz, M.D., Ph.D. Professor of Pathology and Medicine Director, Brander Cancer Research Institute New York Medical College BSB, Room 438 Valhalla, N.Y. 10595 www.darzynkiewicz.com/zbigniew/ <http://www.darzynkiewicz.com/zbigniew/> -----Original Message----- From: Michie, John, Dr <jm5@sun.ac.za> [mailto:jm5@sun.ac.za] Sent: Thursday, December 13, 2007 4:05 AM To: cyto-inbox Subject: PHA stimulation of PBMCs results in sticky cells A colleague has the following problem: After stimulation of PBMCs with PHA the cells become sticky. Trying to analyse on flow shows reduced numbers of cells compared to fresh. Cause of stickiness? Any help flowers? Kind regards John Michie +27 21 9389 539 +27 21 933 8886 083 449 2225
