Date: Fri Dec 14 2007 - 14:59:05 EST
John, PHA causes some cell death which releases DNA which causes cells to stick, presumably via a charge-charge interaction. A 5 minute incubation with 3500 Dornase units/ml of DNAse (in a Mg++ containing buffer!) will liberate them. Calman From: "Michie, John, Dr <jm5@sun.ac.za>" <jm5@sun.ac.za> Date: Thu, 13 Dec 2007 11:05:21 +0200 To: cyto-inbox Conversation: PHA stimulation of PBMCs results in sticky cells Subject: PHA stimulation of PBMCs results in sticky cells A colleague has the following problem: After stimulation of PBMCs with PHA the cells become sticky. Trying to analyse on flow shows reduced numbers of cells compared to fresh. Cause of stickiness? Any help flowers? Kind regards John Michie +27 21 9389 539 +27 21 933 8886 083 449 2225
