Long ago (prehistoric times) we have measured, by quantitative microinterferometry dry mass of individual lymphocytes, unstimulated and stimulated by PHA, published in: Darzynkiewicz, Z., Dokov, V., and Pienkowski, M.: Dry mass of lymphocytes during transformation after stimulation by phytohemagglutinin. Nature, 214:1265-1266, 1967. The dry mass of lymphs increased from ~30 pg to over 400 pg during mitogenic stimulation. One may expect that at least 50% (>15 pg) of dry mass of nonstimulated cells would be protein (much higher proportion is in lymphoblasts), remaining 50% is DNA and RNA. Zbigniew Darzynkiewicz, M.D., Ph.D. Professor of Pathology and Medicine Director, Brander Cancer Research Institute New York Medical College BSB, Room 438 Valhalla, N.Y. 10595 www.darzynkiewicz.com/zbigniew/ -----Original Message----- From: Katarzyna Ruckemann-Dziurdzinska [mailto:kruck@amg.gda.pl] Sent: Wednesday, December 12, 2007 5:40 PM To: cyto-inbox Subject: lymphocyte protein content A few weeks ago there were a few posts regarding lymphocytes volume. I would like to ask - what is an average protein content of a million unstimulaed human lymphocytes - it is if you precipitate them whith PCA, TCA or similar and measure protein in the pellet (Bradford, Lowry - any method) - how many micrograms per million cells should one get? As I understood average lymphocyte volume is around 200 fL. How can one measure this? Has anyone ever came across human lymphocytes that would be bigger (or smaller)? What lymphocyte volume differences would one notice on the FACS scatter? I would be grateful for any comments. Kate -- Dr Katarzyna Ruckemann-Dziurdzińska Katedra i Zakład Fizjopatologii Akademia Medyczna w Gdańsku ul. Dębinki 7 80-809 Gdańsk tel. +48 58 3491511 fax. +48 58 3491510Received on Sat Dec 15 15:45:01 2007
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