Serotec sells Mycoplasma Removal Agent. Not exactly sure of the mode of action, but I have used it extensively on human and murine cell lines with no loss in proliferative capacity or effect on plating efficiency/ clonogenic cell survival following ionizing radiation, cisplatin, or mitomycin C. Usually use it at their recommened dilution of 1/100, and after a couple of weeks, cells are clean based on flourescence detection using Hoechst 33258 staining after fixation with methanol/acetic acid (found in Freshney's Cell Culture text). Best Farid -- Farid Jalali MSc Senior Research Technician/ Lab Manager Dr. Robert Bristow Lab Applied Molecular Oncology Princess Margaret Hospital Toronto, Canada 416-946-4501 X4351 (Princess Margaret Hospital) 416-581-7754 STTARR at MaRS Building 416-581-7791 STTARR Micrroscopy SuiteReceived on Sat Dec 8 16:58:00 2007
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