Hi Vinko Try altering the settings for the "2nd drop, 3rd drop, 4th drop" on the streams panel (that with the drop delay on it - on our software at least), I think the two streams close to the centre stream should then "disappear" into the centre stream. Usually the "2nd drop" has the most influence. Ian Ian Titley PhD Section of Haemato-oncology Institute of Cancer Research 15 Cotswold Road Belmont Sutton Surrey SM2 5NG UK Tel +44 (0)20 8722 4255 >>> "Vinko Tosevski" <vinko.tosevski@medri.hr> 05/12/2007 22:11 >>> Hi, My department recently bought an Aria and I was among the first ones to go through operator's training for it. So, although I have previous flow experience, I am largely a beginner in the sort world. Today I tried to sort some cells using 100u nozzle and LOW sort mode (for the first time; most of the time we use 70u nozzle and HIGH sort mode). The stream looked OK but, when I applied the voltage and used TEST SORT button, I got 6 side streams instead of 4!??! (I will try to attach a picture but I've never seen any attachment going through) Is there a simple explanation to this phenomenon and how can I resolve this? When I move the sliders used to adjust the position of side streams, the outer two side streams on each side respond. But, the center stream splits/gets 2 side streams of its own after applying a voltage, and they are inresposive to the movement of any sliders. These two are not too much apart but are clearly visible as separate streams in camera window... Any comment is more than welcome. Sincerely, Vinko ---- Vinko Tosevski, MMolBiol Research fellow/Teaching assistant, Department of physiology and immunology, Rijeka Medical School, Ul. b. Branchetta 20, HR-51000 Rijeka, Croatia Tel: +385 51 651 192 Fax: +385 51 675 699 The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addressee only. If the message is received by anyone other than the addressee, please return the message to the sender by replying to it and then delete the message from your computer and network.Received on Fri Dec 7 13:58:01 2007
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST
