Re: 6 side streams in ARIA?!?

Hi Vinko,

I guess you must adjust the defanning of the center stream. It's done by
the values in the 2nd, 3rd en 4th drop	windows below your stream
viewing window. 
Try 20, 10, 5.

good luck
Berend Hooibrink
Cellbiology AMC
Amsterdam

----- Original Message -----
From: Vinko Tosevski <vinko.tosevski@medri.hr>
Date: Wednesday, December 5, 2007 11:11 pm
Subject: 6 side streams in ARIA?!?

> Hi,
> 
> My department recently bought an Aria and I was among the first 
> ones to go
> through operator's training for it. So, although I have previous flow
> experience, I am largely a beginner in the sort world.
> 
> Today I tried to sort some cells using 100u nozzle and LOW sort 
> mode (for
> the first time; most of the time we use 70u nozzle and HIGH sort 
> mode). The
> stream looked OK but, when I applied the voltage and used TEST SORT 
> button,I got 6 side streams instead of 4!??! (I will try to attach 
> a picture but
> I've never seen any attachment going through) Is there a simple 
> explanationto this phenomenon and how can I resolve this? When I 
> move the sliders used
> to adjust the position of side streams, the outer two side streams 
> on each
> side respond. But, the center stream splits/gets 2 side streams of 
> its own
> after applying a voltage, and they are inresposive to the movement 
> of any
> sliders. These two are not too much apart but are clearly visible as
> separate streams in camera window...
> 
> Any comment is more than welcome.
> 
> Sincerely,
> Vinko
> 
> ----
> Vinko Tosevski, MMolBiol
> 
> Research fellow/Teaching assistant,
> Department of physiology and immunology,
> Rijeka Medical School,
> Ul. b. Branchetta 20,
> HR-51000 Rijeka, Croatia
> 
> Tel: +385 51 651 192
> Fax: +385 51 675 699
> 
> 
>