Hi, My department recently bought an Aria and I was among the first ones to go through operator's training for it. So, although I have previous flow experience, I am largely a beginner in the sort world. Today I tried to sort some cells using 100u nozzle and LOW sort mode (for the first time; most of the time we use 70u nozzle and HIGH sort mode). The stream looked OK but, when I applied the voltage and used TEST SORT button, I got 6 side streams instead of 4!??! (I will try to attach a picture but I've never seen any attachment going through) Is there a simple explanation to this phenomenon and how can I resolve this? When I move the sliders used to adjust the position of side streams, the outer two side streams on each side respond. But, the center stream splits/gets 2 side streams of its own after applying a voltage, and they are inresposive to the movement of any sliders. These two are not too much apart but are clearly visible as separate streams in camera window... Any comment is more than welcome. Sincerely, Vinko ---- Vinko Tosevski, MMolBiol Research fellow/Teaching assistant, Department of physiology and immunology, Rijeka Medical School, Ul. b. Branchetta 20, HR-51000 Rijeka, Croatia Tel: +385 51 651 192 Fax: +385 51 675 699 This attachment - 'DSC00502.JPG' - 919.53 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/54e17b60744833131d3be63bff34cfc5f7c109b4.JPGReceived on Thu Dec 6 13:58:00 2007
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