Date: Mon Dec 03 2007 - 03:54:16 EST
Hi B., safest is always to trash your contaminated cells and start up from a new cyrovial - if you have that luxury. We ourselves sometimes receive contaminated transfectants/hybridoma's/weird and hard to get cell lines, so there's no chance of going back to a safe in-house stock. In those cases, I treat them at least one week with MRA (from ICN, now MP Bio), then serially with BM cyclin 1 and 2 (from Roche), as instructed by the supplier. The triple-whammy usually does the trick, as monitored by re-emergence of mycoplasma contamination after all treatment is withdrawn for at least one week after the last round. Of course, you should always keep those cells in a separate incubator (where facilities allow), not handle them simultaneous with uncontaminated cells in the same hood,... untill you're really sure they're clean. We have a special "quarantine" incubator for newly arriving cells, which is also capable of sterilizing itself afterwards. Guy Next generation therapeutic antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 262 00 00 <http://www.plaxo.com/click_to_call?src=jj_signature&To=%2B32+(0)9+262+0 0+00&Email=guy.hermans@ablynx.com> +32 (0)9 262 00 01 +32 (0)486 788 551 <http://www.plaxo.com/click_to_call?src=jj_signature&To=%2B32+(0)486+788 +551&Email=guy.hermans@ablynx.com> Want to always have my latest info? <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972&src= client_sig_212_1_banner_join&invite=1> Want a signature like <http://www.plaxo.com/signature?src=client_sig_212_1_banner_sig> this? -----Original Message----- From: Bindu Aramati [mailto:bindiya30@yahoo.com] Sent: Thursday, November 29, 2007 6:17 PM To: cyto-inbox Subject: Mycoplasma Hi, Though it is not related to Flow, but everyone works on cell culture, Must be able to help me here. We come to know there is mycoplasma contamination in our cell and could you please let us know how to clean up & further care and what would have been the adverse effects on cells ! Than you in advance for your time. Bindu Aramati Postdoctoral fellow _____ Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See <http://us.rd.yahoo.com/evt=51732/*http://overview.mail.yahoo.com/> how.
