Dear Flowers, I need your help to figure out what's going on with my 6 color compensation on FACSCanto. As you can see at the plots (word document attached), I have problems to compensate APC x PE-Cy5, is it normal the overlap be that high? I have to compensate more than 70. This is an example of human PBMC labeled with CD44 FITC, CD8 PE, CD4 APC, CD62L PE-Cy5, CD25 PE-Cy7, CD69 APC-Cy7. But almost all my 6 color tubes have the same problem, doesn't matter which antibody I am using. I don't know if there is any correlation, but with some cells, especially DCs, the isotype is always positive for APC and PE-Cy5. Sincerely, I don't know what to do, any help will be really appreciate. Thanks again Ana. Ana Leda F. Longhini, M. Sc. PhD student - UNICAMP - SP - Brazil e-mail: analeda.consultoria@gmail.com This attachment - 'CD4xCD62L.doc' - 30.72 KBytes - can be viewed at http://www.cyto.purdue.edu/MD-parts/d099e412fd7a83d9c61afc0c1fcfa1e6cc0c4550.docReceived on Fri Nov 23 16:38:00 2007
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