Hi all - I have recently started optimizing a phagocytosis assay using those cute, little 1um yellow-green fluorescent beads. I am having a carry over issue. I have run multiple clean-out protocols (lots of warming of reagents, whole tank switchout, cleanse solution) and still I get this blip of junk on my scatter plot. It is below my calibration bead threshold - so I can still pass my calibration, but it is annoying me that I cannot seem to get them all out! Any experience out there??? Thanks in advance!! Sarah -- Sarah E. Leavell Research Associate The Ohio State University College of Veterinary Medicine Department of Veterinary Biosciences 1925 Coffey Road Columbus, OH 43210 614-247-8025 (lab) leavell.8@osu.eduReceived on Wed Oct 24 12:58:00 2007
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