On the Facstar Plus with CellQuest, Joseph Webster used to record a file(s) which only stored events within the sort gates. This approach has some limitations, particularly for very rare event sorts, but in a lot of cases it does provide a nice electronic record of the sort regions. We tried this for a while in Diva but it never worked as well. The biggest problem was that for many versions there was a memory leak that would crop up if you did this and it would often result in the software locking up in the middle of the sort. Not sure if this is fixed in v5. There are also various other limitations that came up, eg not all gate types will allow you to define a storage gate. We stopped doing it because of the memory leak and have settled on either hard-copy printouts and/or pdf prinouts made immediately after the sort. Regards, Adrian On 17/10/2007, at 5:36 AM, Marty Bigos wrote: > That's true. the experiment export does not give you a total > history of your worksheet, only the last settings. One can print > out the worksheet as a visual documentation. However, I do not know > of a way to electronically document the sort (other than by > exporting the experiment right after the sort). If you have any > other ways to do this, I would be interested in hearing about them. > > Marty Bigos, Director > Gladstone Flow Core > > > > On Oct 15, 2007, at 5:46 PM, Adrian Smith wrote: > >> >> On 15/10/2007, at 5:10 AM, Marty Bigos wrote: >> >>> Experiment export is not useful for this. Not only are the file >>> names useless, any changes to the annotation after data >>> collection is not recorded in the FCS file. The Experiment >>> export, however, is very useful for backup and archiving. All >>> plots and gates on the worksheets are kept, providing >>> documentation for sorting that may have been done. >> >> Except that the gates are not "fixed" in any way, ie they may well >> have been moved at some point after the initial sort was started, >> particulalry if people have been looking at the files and >> wondering what might have happened if the gates were moved just a >> bit this way or that... >> >> I prefer a more permanent record of sort gates :) >> >> >>> And this allows users to set up and annotate an experiment fully >>> on stand-alone Diva software on a remote computer, export it as >>> an experiment, and import it on the instrument connected system. >>> This can help throughput in a busy facility. >> >> This is indeed very useful. >> >> Regards, >> >> Adrian Smith >> Centenary Institute, Sydney, Australia >> >> >Received on Wed Oct 17 14:58:01 2007
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST
