Date: Wed Oct 10 2007 - 10:04:40 EDT
Hello Fellow Flowers: I have a question from a client who had done apoptosis studies using 7aad. We are seeing two populations that are 7AAD positive. Is it possible to distinguish if one population is early apoptosis and one is late apoptosis? If that is possible, how do we distinguish the late apoptosis from the necrotic cells? I have included a copy of one of the data files-now that we look at it-is it that the two brightest peaks represent late apoptosis and necrosis? As you can tell we really need some assistance in interpreting this data. As always, thanks in advance. And for those who are wondering, I am still compiling data on the rates charged by everyone. I keep getting interrupted by pesky clients wanting to run experiments for flow and sorting. Why won't they leave me alone!!! Janet -- Janet Dow Research Specialist and Manager Flow Cytometry and Cell Sorting Facility North Carolina State College of Veterinary Medicine 4700 Hillsborough Street Room C-309 Raleigh, NC 27606 (919)513-6443
