Dear Flowers, Thanks for all the replies to my question of last week concerning 12um pore size transwell plates. I've put all replies in this email for other peoples benefit. We are going to test Millipore standing inserts to see if they can be used for our experiments Floris Floris van Alphen Sanquin CLB Dept. Molecular Cell Biology / Flow Cytometry Plesmanlaan 125, 1066 CX Amsterdam The Netherlands +31(0)20-5123347 F.vanalphen@sanquin.nl Hi Floris, The BD 8um pore size plates ( 24 well inserts) are a good alternative as they still allow cells to go through if you are looking at the pore size for a metastasis assay. best regards Parul Parul.Sharma@childrens.harvard.edu Hi, Check Nunc. Dan dfreeck@guavatechnologies.com Check with Millipore has all sorts of transwell inserts including 12um. http://www.millipore.com/catalogue.nsf/docs/C10504 I hope this helps... Ali Borazjani Biomedical Engineering Dept. Ag & Bio Engineering Life Sciences and Biotechnology Inst. Mississippi State, MS 39762 Ph (662)312-7601 Hallo Floris: We use Neuroprobes chambers (12 wells) with polycarbonate or cellulose nitrate filters. T. Parra Cid Unidad de Investigaci¨®n Hospital Universitario de Guadalajara Hi Floris, Greiner en BD (Falcon) hebben vergelijkbare platen, evenals Millipore. Falcon had recent enkele *maanden* geen platen beschikbaar, wat ons in vergelijkbare problemen bracht. Je student(e) moet er erg op letten dat deze producten vergelijkbaar maar niet identiek zijn. Zelfs al mocht je dezelfde fitlermaterialen en poriegrootte vinden (onwaarschijnlijk), dan nog zullen de performance karakteristieken anders zijn. Guy Guy.Hermans@ablynx.com Floris, Dit vind ik met google: Tissue Culture Inserts (12 Well) - Biocompare Buyer's Guide CORNING TRANSWELL MEMBRANE, PC, 12MM DIAM, 12UM PORE, TC-TREATED, STERILE Sigma-Aldrich, Material: polystyrene polycarbonate membrane Pore Size: 12.0 ¦Ìm ... www.biocompare.com/matrix/13868/Tissue-Culture-Inserts-(12-Well).html - 55k Maar dit zul jezelf ook wel gevonden hebben. Groeten Arie van der Marel vandermarel@distrilab.nl Dear Dr. van Alphen, I thank you for your request below. I inform you that we do not have any 12um pore size inserts. However, we do sell a 12-well insert with 8um pore size that we recommend only for very big cells (like the tumor cells). In general what matter is the nucleus size and not really the cell size. The nucleus is less flexible than the cytoplasm and constitutes the limiting factor for a cell to go through the pores. Can I ask you which kind of cells you are planning to use? I hope this will help a little. Best regards, Nathalie Nathalie Suarez Gonzalez, PhD BD Biosciences Scientific Support Europe Tel: (+31) 010/711.48.00 Fax: (+31) 010/711.48.01 e-mail: help.biosciences@europe.bd.com http://www.bdbiosciences.com/ Hi Floris, want to remember we used them from Falcon while ago, not sure about the pore size but you can check in their website. Hope it helps, Lola Lola.Martinez@cancer.org.uk Hello Floris, have a look at Greiner Bio one, perhaps they have asimilar product. Grit grit.schade@leister.com *******************************DISCLAIMER****************************************** Gebruik van deze informatie door anderen, te weten de niet geadresseerden,zonder toestemming van de afzender of de geadresseerde(n) is onrechtmatig. Sanquin is niet aansprakelijk voor onjuiste of onvolledige overbrenging van de inhoud van een verzonden e-mailbericht, noch voor de late ontvangst daarvan. Use of this information by persons other than the addressees, without the sender.s or the addressee(s). permission, is unlawful. Sanquin is not liable for any incorrect or incomplete conveyance of information contained in an e-mail message sent, nor for its delayed reception. *******************************DISCLAIMER******************************************Received on Mon Sep 24 12:18:00 2007
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