Re: DiI and FITC on a Vantage

On 06/08/2007, at 7:59 AM, Ray Hester wrote:

> However, if we excite DiI with a 530nm line (which should result in  
> brighter fluorescence, again, according to Mol. Probes) from the  
> Spectrum laser, it seems to cause problems with detection of FITC,  
> almost as if some of the 530 nm laser light	is being detected by  
> the FITC FL1 PMT.

That wouldn't be surprising. I believe it can be a problem on some  
LSR-IIs with 532nm lasers. On our FACSVantageDiva we can certainly  
get scattered 590nm light from our dye laser into our PE channel if  
we are not careful with the alignment and filters.

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia