different antibody/dye - difference in the read out?

From: Anbu Karuppannan <anbukarup@gmail.com>
Date: Sat Jul 21 2007 - 00:37:56 EDT
Hi all,

I am new to flow cytometry. I am staining swine PBL for surface
markers, (fix and stain) i want to know if different antibodies/dyes show
difference in the intensity of fluorescence for the same surface marker. I
am using primary ab directly conjugated to dye. How can i go about this
factor?

Anbu
Received on Mon Jul 23 12:38:00 2007

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