Multiplex Bead Assays

From: Andrew Beernink <Andrew.Beernink@Dako.com>
Date: Wed Jul 11 2007 - 12:09:21 EDT
Hello-
 
I have a client who wants to set up multiplex bead assays for soluble
analytes.
 
Some of the commercial kits will prove useful, but said client would
also like the ability to use his own antibodies.
 
1.  Can anybody provide a protocol for conjugating Ab to beads?
Spherotech was kind enough to provide some bead samples free of charge
(with and without a carboxyl moiety).  Luminex has a protocol on their
website, but the link is faulty (as are a LOT of their links).
 
2.  Will Luminex bead sets work on current 405/488/635 cytometers?
Alas, links to their instrument specs are also a bit whacked.
 
Thanks for any feedback.
 
Andrew
 
Andrew Beernink
Senior Applications Specialist
Dako Colorado
(858) 353-7007
(858) 435-1137 efax
Received on Thu Jul 12 11:58:00 2007

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