Hello everyone. I have a client who would like to sort live cells that are proliferating the most in response to an infection and then perform an adoptively transfer. Is there an easy way to do this? Some suggestions I've had so far involve purchasing a mouse strain that produces GFP as part of its histone proteins, or feeding the mice enough CFSE to enable sorting of highly proliferative cells after the infection period (4 weeks). I would really appreciate more suggestions or advice. Thanks for your contributions. Kylie Price Flow Cytometry Manager The Malaghan Institute of Medical Research The Central Services Building Victoria University Campus Entrance 7 Kelburn Parade Wellington 6242 New Zealand Ph: 64-4-4996914, ext 850 Fax: 64-4-4996915 E-mail: kprice@malaghan.org.nz Web: www.malaghan.org.nz
This archive was generated by hypermail 2.1.8 : Wed Jan 31 2007 - 03:12:00 EST