Date: Thu Jun 14 2007 - 11:11:25 EDT
Dear Flow Cytometry Community, I have a question about filter configurations for Qdots in flow cytometry. I would like to order a new instrument with a violet laser to measure the full range of qdot fluorochromes. From quick talks with BD, I have a list of the filters they suggest. They represent slightly narrower signal ranges in just about all cases than the filters used by the fellows in the paper by Chattopadhyay et al in Nature Immunology last year. For those of you working with these reagents in flow, could you offer your opinion on this matter? Would you recommend collecting more signal (and probably doing a bit more 'inter-qdot' compensation) or would you suggest taking the narrower band pass, collecting less signal and needing less compensation? Here is a list of the bandpass filters suggested from BD as well as those used in the paper mentioned above. Chattopadhyay et al Suggestion from BD QDOT800 780/60 (750LP) 800/30 QDOT705 705/70 (670LP) 710/50 QDOT655 660/40 (640LP) 660/20 QDOT605 605/40 (595LP) 610/20 QDOT585 585/42 (570LP) 585/42 QDOT565 560/40 (557LP) 560/40 QDOT525 515/20 525/50 Any and all recommendations, ideas and advice are appreciated. Thanks a lot to the organisers and moderators who run this list and all the people who take the time to dish out their 2 cents- it is great! Best wishes, Joanna Flow Cytometry Core Facility EPFL, Swiss Federal Institute of Technology SV-SG, Station 15 CH 1015, Lausanne Switzerland +41 21 69 39 547
