Re: Dynabeads problems

Hi Li,
When most of the magnetic beads companies make a tall claim of >90% purity 
and >90% recovery, in practice it turns out much lesser than that.
Kinldy go through attached e-mail threads, which ran a year back (!), on the 
same forum,  to find out the truth!

Best Regards, Hemant

----- Original Message ----- 
From: "chen li" <chen_li3@yahoo.com>
To: cyto-inbox
Sent: Thursday, May 03, 2007 3:18 AM
Subject: Dynabeads problems


> Dear all,
>
> I recently use sheep anti-mouse Dynabeads  from
> Invitrogen to perform negative selection on human
> PBMCs. I counted the cell number of both fragments
> under microscope after separation. I got pretty good
> recovery of negative fragments but very poor recovery
> of positive fragment.Last time I began about 3x10e6
> negative cells and 7x10e6 positive cells before
> separation (checked by FLOW) I got about 2.0-2.86
> x10e6 cells from the negative portion BUT only
> 1.5x10e6 cells from the positive fragments and I LOST
> about 5.5x10e6 cells from the positive cells. Although
> my interest right now is negative fragment I would
> like to know what happened to the positive fragment.
> Another question: Does Dynabeads affect cell's
> functions or growth? I saw my cells of negative
> fragment didn't grow very well, which is contrary to
> what is said in the data sheet.
>
> Any idea or suggestion will be appreciated,
>
> Li
>
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