Kerry In your posting you refer to 2N cells that are in G2/M phase and 4N cells in G0. We typically refer to the 2N (or 2C) in G2/M as DNA Diploid G2/M and the 4N (or 4C) events as DNA Tetraploid G0G1 events. There is no direct way to tell the difference between a DNA Diploid G2M event and a DNA Tetraploid G0G1 events in single-parameter DNA analysis. They both produce the same fluorescence signal which is counted in the same channel of the cytometer. Your observation that to call something "DNA Tetraploid" requires identifying other parts of the " DNA Tetraploid" population is a good one. We have established a rule set for evaluating DNA cell cycle histograms and the presence of 6C and 8C peaks is one of the decisions points. I would be happy to send you a copy of the rule set if you are interested. Best regards, Don Donald J. Herbert Technical Support Manager Verity Software HouseReceived on Thu May 3 14:38:00 2007
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