Hello All, A researcher in our institute would like to observe FRET in the following way. I want to observe FRET between conjugated antibodies. The donor fluorochrome being Alexa 555 and the acceptor fluorochrome Alexa 647. Therefore would need to excite the Alexa 555 and observe emission from the Alexa 647. We have a FACSAria and a FACSVantage with the possibility of 514 excitation. Has anyone done anything similar or can you help as to how this can be achieved. Mike Hughes, Flow Cytometry, PICR, Manchester, UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.Received on Tue Apr 24 11:58:00 2007
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