Date: Fri Apr 20 2007 - 16:00:38 EDT
Speaking of streams elongating, I've been wondering why mine does and what I should do about it. Aria Flowers recognize the elongation as an increase in the Drop 1 value on the sorting window. If the Drop 1 increases enough, then the sweet spot ( auto pilot) starts flickering as it trys to keep the Gap value constant but doesn't know where to set the break off. I had been guessing that the elongation was a result of a pressure increase, but a service rep recently told me that he once hooked up a digital pressure meter to the port on the side of the unit and it didn't change, even though the Drop 1 kept going up through the day. I guess the temperature theory makes more sense, but still there's the issue of what to do about it. If your sweet spot is flickering, you've got to do something about it because it shuts down sorting while it's happening. You can bring back the Drop 1 by 1) altering the phase 2) lowering the pressure 3) increasing the frequency. Otherwise, you shut down sorting and re calculate your drop delay, resterilize and then resume. Altering the phase isn't the answer. I'm pretty sure that brings it back by altering the strobe timing. Your sweet spot won't flicker , but your charging will be off. Does any one have a feel for whether you should adjust the pressure or the frequency? Dan Rosson Ph.D. Flow Jock University of Tennessee PS If you would like me to forward your email address to one of those spam sites, send me an out of office reply. ________________________________ From: akos.szilvasi@novartis.com [mailto:akos.szilvasi@novartis.com] Sent: Wednesday, April 18, 2007 1:58 PM To: cyto-inbox Subject: Re: Air Conditioning for Sort Lab Hi, Ultan, You must have steady temperature for the MoFlo. That sorter is sensitive to the room temperature changes. The drop delay will move, the stream elongates as the room warms up....and the room will warm up when you operate them because of the heat output of the different components. Make sure - if you need to have water cooled lasers - that the heat exchanger is outside of the flow room. However, a 40 sm space has a big temperature buffering capacity that may not cause problems. Best regards, Akos __________________________ Akos Szilvasi NIBRI Core Laboratory Services manager USCA, 601-5301 Novartis Institutes for BioMedical Research, Inc. 100 Technology Square Cambridge, MA 02139 USA Phone: +1 617 8717177 Email : akos.szilvasi@novartis.com <mailto:akos.szilvasi@novartis.com> Ultan Cronin <ultan.cronin@ul.ie> 04/18/2007 03:32 PM To Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc Subject Air Conditioning for Sort Lab Dear Flowers, We will soon be installing an air conditioning system in our lab, which contains one BD LSR I and a MoFlo. The lab has one door, three large windows and would be about 40 meters sq. Has anyone any advice to offer us before we commit a design to paper? Yours gratefully, Ultan -- Ultan Cronin BSc MSc Dept of Life Sciences Schrodinger Building University of Limerick Castletroy Co. Limerick Ireland Tel: 00353-(0)61202890 Mob: 00353-(0)872032963 Fax: 00353-(0)61331490 _________________________ CONFIDENTIALITY NOTICE The information contained in this e-mail message is intended only for the exclusive use of the individual or entity named above and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivery of the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail and delete the material from any computer. Thank you.
