Date: Fri Apr 20 2007 - 04:00:19 EDT
Hi Brian, as a general rule, unpaired heavy or light immunoglobulin chains are quite insoluble and prone to aggregation/precipitation when forced into that form (i.e. reducing and blocking free cysteins before they can re-oxidize together again). I heard you can keep it in solution in acetic acid or so, but not in more regular assay buffers. Therefore, you're probably stuck with using intact i.e. kappa light chain monoclonals and using the lambda isotype control for counterscreening, or you could reduce some immunoglobulin, separate under reducing conditions (gel filtration, preparative SDS-PAGE come to mind) and just deal with fact you won't have functionally folded material that way. Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Brian Lee [mailto:brian@immunochemistry.com] Sent: Wednesday, April 18, 2007 10:19 PM To: cyto-inbox Subject: Commercial Source: Mouse kappa and lambda proteins Hello Everyone, I was hoping that someone out there in the Cytometry Mailing List group would know a commercial source for purchasing purified mouse Kappa and Lambda proteins. I am developing an in-house ELISA for isotyping purposes and wanted to get some of these proteins to use as assay standards. I have looked seemingly everywhere and one can find numerous sources of mono and polyclonal antibodies to these two light chain proteins but I have been unable to find a source for the actual proteins themselves. If any of you know of any source where these proteins can be purchased I would greatly appreciate hearing from you. Thanks in advance and have a great day, Brian Brian W. Lee Ph.D Vice President Immunochemistry Technologies, LLC 9401 James Avenue South, Suite 155 Bloomington, MN 55431 USA 800-829-3194 952-888-8788 fax 952-888-8988 brian@immunochemistry.com ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
