Date: Thu Mar 29 2007 - 14:20:34 EDT
Elizabeth, I have used several of the FACSFlow systems for a long time. We had no problem with any of the units. They are reliable and need practically no maintenance. Despite of my positive experience with the FACSFlow I would not buy it again. There is a better solution for the LSR II. BD offered us 20 liters steel tanks. This is the same tank as the regular LSRII tank but much bigger. Answering your other question, yes, the HTS uses a lot of sheath fluid because the sheath fluid serves as the wash and rinse agent to the sampler. This is very convenient for not having to monitor yet an other container's liquid level. Actually we switched to distilled water for simplicity and less complication with salt deposits. In order to monitor the water level in the tanks we put them onto a bathroom scale. Once you calibrate the scale (read the weight of the empty tank and the full tanks) you know when to fill it up. We have not noticed any pressure instability with low level of water in the sheath tanks. Akos __________________________ Akos Szilvasi NIBRI Core Laboratory Services manager USCA, 601-5301 Novartis Institutes for BioMedical Research, Inc. 100 Technology Square Cambridge, MA 02139 USA Phone: +1 617 8717177 Email : akos.szilvasi@novartis.com "Kraus, Elizabeth" <elizabkr@BaylorHealth.edu> 03/29/2007 01:56 PM To Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> cc Subject LSR II FACSFlow system Hello all... Please let me hear from you if you have an LSR II with the FACSFlow and HTS Systems. My questions are: 1) Would you purchase the FACSFlow System again? 2) Does the HTS use more sheath than regular tubes? For those of you with the standard sheath system... How long does a tank last if running samples all day long? Thanks in advance! Elizabeth Elizabeth T. Kraus Manager, Flow Cytometry core Facility Baylor Institute for Immunology Research 3434 Live Oak, Suite 200.1 Dallas, TX. 75204 214-820-3586 elizabkr@bhcs.com -----Original Message----- From: Julie Bertout [mailto:julie.bertout@ibl.fr] Sent: Wednesday, March 28, 2007 8:58 AM To: cyto-inbox Subject: lymphocyte cell cycle analysis Hello, Does anybody have a protocol to study cell cycle of lymphocytes purified from blood? I suggested the regular PI staining I used for cell line - fixation with cold EtOH 80%, - storage at 4°C, - stain 1 million cells with 100ug IP and 125ug RNase 30min @ 37°C but the morphology of the cells completely changed after this treatment. The lymphocyte were quiescent. The researcher did a positive control using PHA to induce cell cycle but the populations changed even more... Thank you for your advices. Julie Bertout cytometry lab Institut Pasteur de Lille 1 rue du professeur Calmette 59800 Lille France This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _________________________ CONFIDENTIALITY NOTICE The information contained in this e-mail message is intended only for the exclusive use of the individual or entity named above and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivery of the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail and delete the material from any computer. Thank you.
