RE: Stem Cells and violet laser

 

Hi Paolo,

 

I have tried to detect side population on our FACS Aria (with violet laser not UV) with Hoechst and the excitation was minimal.  However, I was able to see SP in mouse bone marrow and some cancer cell lines using DyeCycle violet (from Invitrogen).  However, the resolution was not as good as FACS Vantage when compared in parallel using an UV source.  I also heard that the UV laser on FACS Aria is not powerful enough for SP detection but I have not validated that myself. 

 

Just my 2c.

 

Albert Tai

Exelixis, Inc. 

________________________________

From: Cappella, Paolo Elia [Nervianoms] [mailto:Paolo.Cappella@nervianoms.com] On Behalf Of NMS, Svc_facs [Service]
Sent: Monday, February 26, 2007 7:02 AM
To: cyto-inbox
Subject: Stem Cells and violet laser

 

 

Dear Flow-ers,

our institute have planned to buy a new FACSorter. We are oriented to BD FACSAria for several reasons. Our business involved also identification and sorting of stem cells by side population using Hoechst.

Now we are using a FACSVantage with Enterprise laser. 

Italian BD sales offices say that a custom FACSAria with UV laser could be cost up to 400K Euro if we replace violet standard instead around 350K Euro for standard configuration.

My question is : is it possible identify Hoechst SP by Violet or is it difficult to use? 

 

Additional question is: is it possible and is it easy use FACSAria as only analyzer instead to sorting?

 

Another question is: are there other problems that i have to take in mind before buy form BD ? 

 

I put several meats on fire (italian popular rule, i don't know if is it used in your country...). 

 

I Regard to all Flow Cytometry people to reply to my requests.

 

Of course you can reply to me also directly. Thanks.

 

Dr. Paolo Cappella 
Unità di Citometria a Flusso 
Dipartimento di Biologia 
Nerviano Medical Sciences srl 
Via Pasteaur 10 - I 20014 Nerviano MI
tel. +390331581367/3389003111 
fax +390331581233 



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