Hello Flowers, BD sells "QuantiBrite PE beads" that are calibrated according to the number of PE molecules bound per bead. In the old days, BD used to sell PE-conjugated antibody reagents that were specially manufactured so as to have "guaranteed" one PE molecule per antibody molecule (F:P = 1). These specific reagents worked with the beads to calculate ABCs --- because of the known F:P ratio. I cannot find these specific antibody reagents listed in the BD catalog nor I could I get any information about them from BD technical support. Can someone tell me whether, these days, any PE-conjugated antibody will do? I don't really trust these ABC calculations , so perhaps the number of antibodies calculated to bind per cell is so rough that the exact F:P ratio doesn't really matter.... Alice Alice L. Givan, Director Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, NH 03756 USA tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu www.dartmouth.edu/~celllabReceived on Fri Feb 16 15:38:00 2007
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