30th Annual Basic Flow Cytometry Course

Dear FLOWers:

The National Flow Cytometry Resource at the Los Alamos National 
Laboratory will be hosting the 30th Annual Research Course in Flow 
Cytometry.  Detailed information on the course content is provided 
below.	The course fee of $1,700 includes a welcoming reception on 
Saturday evening, lunches during the week, two evening sessions with 
refreshments and a banquet on Thursday evening.  Complete information 
is available on the NFCR home page at: http://nfcr.lanl.gov/.  More 
information and application materials can be obtained from Ms. Ruby 
Archuleta, National Flow Cytometry Resource, Mail Stop M888, Los 
Alamos National Laboratory, Los Alamos, NM 87545 [voice: 
505/667-3912, FAX: 505/667-2891, email: rubyra@lanl.gov].

Jim Freyer

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30th ANNUAL RESEARCH COURSE IN FLOW CYTOMETRY

Los Alamos, New Mexico, June 9-15, 2007

Sponsored by:  The National Flow Cytometry Resource (NFCR) and Verity 
Software House

Course Description:  A methods course in flow cytometry will be 
offered with special emphasis on applications in cell biology, 
immunology and screening.  The faculty consists of distinguished 
members of the flow cytometry community.  This course will be aimed 
toward individuals with several years of experience in flow 
cytometry; very little basic material will be presented.  Each 
participant will receive extensive lecture notes and laboratory 
hand-out materials.  Course lecturers and their presentation subjects 
are listed below.  In addition, there will be two informal evening 
sessions, one on Biosafety/Core Operator issues and one on 
state-of-the-art flow instrumentation at the NFCR.  Finally, there 
will be a Special Lecture on applications of flow cytometry to 
analytical chemistry by Dr. Norman Dovichi of the Department of 
Chemistry, University of Washington.

#	Lecturer:			Subject

1.	John Martin		Basics of Flow/Data Analysis
2.	Alan Waggoner		Fluorescent Probes & Fluorescence Detection
3.	Alice Givan		DNA Analysis
4.	Bruce Bagwell		Advanced Data Analysis
5.	Vincent Shankey 	Analysis of Intracellular Epitopes
6.	William Telford 	Apoptosis
7.	John Nolan		Assays for Screening & Discovery
8.	Joseph Trotter		Sorting
9.	Charles Goolsby 	Flow in Patient Management
10.	David Gailbraith		Fluorescent Proteins in Flow & Imaging
11.	Scott Cram		Chromosomes
12.	Carlton Stewart 	Immunophenotyping
13.	Larry Sklar		Cellular Screening Assays & HT Flow
14.	Gerhard Nebe-von-Caron	Microbiology
15.	Steven Graves		Advanced Flow Cytometry Instrumentation


Laboratory Sessions will address sample preparation, instrument 
construction, data collection and analysis, common and more cutting 
edge flow assays, and typical problems encountered in flow cytometry. 
Lab sessions will be hands-on, with plenty of time and opportunity 
for interaction with the instructors.  Lab sessions will utilize a 
variety of commercial and developmental instruments, and 
representatives from several of the commercial vendors will be 
available to demonstrate and discuss their most recent instruments 
and techniques.  Each participant will be given the choice of 
attending six laboratory sessions chosen from the topics below:

#	Instructor				Subject

1.	Martin/Wilder/Jett			Build a Flow Cytometer
2.	Bagwell 			Data Analysis
3.	Givan				DNA Analysis
4.	Stewart/Stewart 		Immunophenotyping
5.	Goolsby/Shankey 		Multiparameter Cell Cycle
6.	Telford 			Apoptosis
7.	Nolan				Microsphere Arrays
8.	Trotter 			Sorting
9.	Galbraith/Hawley			Fluorescent Protein Analysis
10.	Cram				Chromosome Analysis & Sorting
11.	Edwards/Pavlik			High Throughput Screening
12.	Nebe-von-Caron			Microbiology


-- 
James P. Freyer, Ph.D.

Mail Stop E535
Bioscience Division
Los Alamos National Laboratory
Los Alamos, NM 87545
Tel: 505-667-8229
Fax: 505-665-4637
freyer@lanl.gov