Hi Dear Lisa

I'm Elham from Dr.Ghahary's lab.Unfortunately today I can't find you.If you remember I'd like doing flow cytometry for my microspheres.They are gelatin microspheres.I want to know if I incubate with BSA-fitc ,How much BSA-FITC will absorb to them.But the problem is that gelatin itself has fluorescence in most wavelenghth specially fitc one.I attached the fluorescence photos from gelatin microsphere and gelatin microsphere+BSA-FITC.Is it possible to consider gelatin microspheres as a negative control.it's better for me not to change the dye(fitc).

I'll be so appreciated if you help me

Have a nice day

Elham