Brenda, we use cocktails all the time. You're right, the problem of quenching occurs whether you mix them as a cocktail or add them separately. I'm in a research environment but we validate this problem when we titer the antibodies. Once the cocktail is made, we only worry about precipitation over time and periodically check to see that they're still staining at the same intensity. > ---------- > From: Rabeno, Brenda > Sent: Wednesday, December 20, 2006 12:21 PM > To: Cytometry Mailing List > Subject: Antibody cocktails > > Hello all, > > I was recently cited during a CAP inspection for not validating the use of antibody cocktails in accordance to question FLO.21000. We have been successfully using cocktails since we started running leukemia/lymphoma panels three years ago, but the inspector informed me that we needed to validate that no quenching was occurring. > > He suggested that we pipet all the antibodies individually into the tubes and run them in tandem against a sample using our cocktails, but I'm not sure if that will prove the point since the antibodies will still be together in the tube. Is anyone willing to share their procedures or experiences for validating the use of antibody cocktails? Any help would be much appreciated. > > Thanks, > Brenda > > Brenda Rabeno, MT (ASCP) > Flow Cytometry/Tissue Procurement Laboratory > (302) 733-3438 > brabeno@christianacare.org > > > >Received on Thu Dec 21 12:18:01 2006
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