Antibody cocktails

From: Rabeno, Brenda <BRabeno@Christianacare.org>
Date: Wed Dec 20 2006 - 12:21:36 EST
Hello all, 

I was recently cited during a CAP inspection for not validating the use of
antibody cocktails in accordance to question FLO.21000.  We have been
successfully using cocktails since we started running leukemia/lymphoma
panels three years ago, but the inspector informed me that we needed to
validate that no quenching was occurring.  

He suggested that we pipet all the antibodies individually into the tubes
and run them in tandem against a sample using our cocktails, but I'm not
sure if that will prove the point since the antibodies will still be
together in the tube.  Is anyone willing to share their procedures or
experiences for validating the use of antibody cocktails?  Any help would be
much appreciated.

Thanks, 
Brenda

Brenda Rabeno, MT (ASCP)
Flow Cytometry/Tissue Procurement Laboratory
(302) 733-3438
brabeno@christianacare.org
Received on Wed Dec 20 14:18:00 2006

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