I was wondering if anyone might have advice on filter selection to improve the discrimination of weak GFP expression and autofluorescence. We're using an EPICS XL with a 488 laser on mammalian cell lines expressing emerald GFP (peak excitation 487 nm, peak emission 509 nm). The filters we're currently using for this are a 550 dichroic longpass a 525/15 bandpass. Is there room here for improvement? Thanks, Chris ........................................... Christopher Logg University of California, Los AngelesReceived on Fri Dec 15 11:38:00 2006
This archive was generated by hypermail 2.1.8 : Tue Dec 19 2006 - 03:12:07 EST