better filters to distinguish GFP from autofluorescence?

From: Christopher Logg <logg@ucla.edu>
Date: Fri Dec 15 2006 - 05:34:56 EST
I was wondering if anyone might have advice on filter selection to improve 
the discrimination of weak GFP expression and autofluorescence.  We're using 
an EPICS XL with a 488 laser on mammalian cell lines expressing emerald GFP 
(peak excitation 487 nm, peak emission 509 nm).

The filters we're currently using for this are a 550 dichroic longpass a 
525/15 bandpass.  Is there room here for improvement?

Thanks,
Chris

...........................................
Christopher Logg
University of California, Los Angeles 
Received on Fri Dec 15 11:38:00 2006

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