Hello Alice, I had the same problem with my Aria. It turned out that I needed a new transducer-talk to you field service engineer about getting a new one. I have not had the problem since. Hope you have a service contract! Dale Hirschkorn Blood Systems Research Institute 270 Masonic Ave. San Francisco, CA 94118 (415) 749-6672 dhirschkorn@bloodsystems.org www.bloodsystems.org <http://www.bloodsystems.org/> -----Original Message----- From: Furgeson, Alice N [mailto:Alice.N.Furgeson@pfizer.com] Sent: Wednesday, December 06, 2006 9:17 AM To: cyto-inbox Subject: FACSAria unstable drop 1 and gap Hello, I was wondering if any Aria users have experienced unstable drop 1 and gap even with the Sweet Spot on. I use a 100um nozzle. I have de-bubbled the filters, cleaned the flow cell with contrad 70, sonicated the nozzle and changed the O-ring. I have also tried using a different 100um nozzle and a 70um nozzle. The gap still fluctuates between 2 and 20 and the drop 1 goes between 100 to 300. The numbers just bounce everywhere. Any suggestion for why this is happening and any solution? Thanks, AliceReceived on Fri Dec 8 13:18:00 2006
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