RE: FACSAria unstable drop 1 and gap

Alice:

 

Have you played with either the sheath pressure or the frequency?  At
low pressure, I've found I can get a very stable stream at 25 PSI, and a
frequency of 32.  At high pressure, I with a frequency of 92-94.  

 

Have you examined the nozzles with a microscope to make sure that
everything is fine, no pitting or corrosion.

 

Regards

Tim

 

________________________________

From: Furgeson, Alice N [mailto:Alice.N.Furgeson@pfizer.com] 
Sent: Wednesday, December 06, 2006 12:17 PM
To: cyto-inbox
Subject: FACSAria unstable drop 1 and gap

 

Hello, 
I was wondering if any Aria users have experienced unstable drop 1 and
gap even with the Sweet Spot on.  I use a 100um nozzle.  I have
de-bubbled the filters, cleaned the flow cell with contrad 70, sonicated
the nozzle and changed the O-ring.  I have also tried using a different
100um nozzle and a 70um nozzle.  The gap still fluctuates between 2 and
20 and the drop 1 goes between 100 to 300.  The numbers just bounce
everywhere.  Any suggestion for why this is happening and any solution?

Thanks, 
Alice