Fwd: Would you please forward my question to the Purdue cytometry maillist?

Is it possible to help out this person?

Begin forwarded message:

> From: "Wang, Xin-Xiang" <WangXX@MedImmune.com>
> Date: November 19, 2006 1:22:54 PM PST
> To: lbarsky@chla.usc.edu
> Subject: Would you please forward my question to the Purdue  
> cytometry maillist?
>
> Dear Dr. Barsky:
>
> Sorry that I am bothering you.  I cannot subscribe to the Purdue  
> Flow Cytometry Email list due to some email problems.  Would you  
> please forward my question to the email list?  Thank you very much!
>
> Sincerely,
> Xinxiang
> ~~~~~~~~~~~~~~~~~~~~~~~~
> Hello,
>
> I recently encountered a weird phonomenon.  After staining of yeast  
> spheroplast, only 3% of the cells were positive in terms of cell  
> surface protein expression, as determined by	 BD LSRII.  But 30%  
> were positive when the exact samples were run on Guava EasyCyte  
> Plus!  I wonder what causes such great difference between two flow  
> cytometers and which machine I should trust.	My instrument  
> settinges seeminly were correct because both machines detected both  
> positive and negative cells.	The difference is the fraction of  
> positive/negative population.
>
> Please email me directly at wangxx@medimmune.com if you have any  
> hints.  Thanks!
>
> Sincerely,
> Xinxiang Wang
>
> ******************************************
> Scientist I
> MedImmune Inc.
> One Medimmune Way,
> Gaithersburg, MD, 20878
>
> WangXX@Medimmune.com
> Tel:	(301)398-4517