Greetings everyone: I have an investigator who is attempting to extract nuclei from unfixed postmortem human cortex and isolate neuronal nuclei from those of other cell types (astrocytes and oligodendrocytes) by sorting. The methylation patterns of the genomic DNA from these neuronal nuclei will then be analyzed. Does anyone have any tips or wisdom they can add with processing the cells for sorting? Any thoughts would be appreciated. Best regards, -Rich Richard F. Konz, Jr. Assistant Professor Department of Medicine Graduate Faculty, Immunology and Virology Program Director, Flow Cytometry Core Facility UMass Medical School 55 Lake Avenue North Room: S5-322 Worcester, MA 01655 Office: 508-856-1598 Lab: 508-856-3276 Fax: 508-856-2755 Pager: 508-722-0560 Email: Richard.Konz@umassmed.edu Lab Web: http://www.umassmed.edu/facslab Faculty Web: http://www.umassmed.edu/ivp/faculty/konz.cfmReceived on Fri Nov 17 11:38:00 2006
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