Hi Alfonso, A few remarks: * does he want to keep the cells intact, or is the filtrate the component he's mostly interested in? Any standard 0.22 micron filter-top bottle will do (see millipore, whatman...) * if the cells are in a medium containing 10% serum or higher, or some other protein, you can probably stop worrying about protein absorption - most filter materials won't absorb detectable amounts of a protein of interest (say, cell-secreted protein your customer may be interested in for measuring/purifying purposes) * to get the most relevant feedback, please state the throughput needs (volume to be filtered, as well as number of samples to be processed in parallel)- there's a lot of filtration solutions out there, but none does it all! Cheers, Guy ------------------------------------ Ablynx NV Guy Hermans, PhD Senior Scientist guy.hermans@ablynx.com Technologiepark 4 B-9052 Zwijnaarde Belgium tel: +32 (0)9 261 06 57 fax: +32 (0)9 261 06 27 mobile: +32 (0)486 788 551 ------------------------------------ -----Original Message----- From: Alfonso Blanco [mailto:alfonso.blanco@ucd.ie] Sent: Monday, November 13, 2006 3:32 PM To: cyto-inbox Subject: membrane filters Dear all, Once again I need your help, this time one of my costumers need to choose a membrane filter for filtration of cells such that all the media or other solutions which will be used in this process get filtered through leaving cells on the membrane. Their Requirements for the filter are:  Negligible Adsorption/Absorption  Low Binding Characteristics  Naturally Hydrophillc  Captures all particles on the surface The membrane filters which I figured out based on the literature review are: * Polyester PETE Membrane * Polyethersulfone Membrane * Polycarbonate Trach Etch Membrane * PVDF Membrane * Glass Fibre Membrane * Cellulose Acetate He thinks that the first three works best in terms of negligible adsorption/absorption and low protein binding. I would be really grateful if anybody can help them to select the best suitable membrane for filtration of cells with these characteristics based on their usage and experience. Kind regards, Alfonso ------------------------------ Dr. Alfonso Blanco Fernández Flow Cytometry Core Facilities UCD - Conway Institute of Biomolecular & Biomedical Research University College Dublin Belfield, Dublin 4 IRELAND T: 00353(0)1 716 6836/6947 ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------Received on Tue Nov 14 12:18:01 2006
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