Date: Fri Oct 27 2006 - 13:27:06 EDT
Hi E., I've played with two intact membrane-crossing b-gal substrates from Mol Probes quite a while ago ("Imagene Green", "Imagene Red"). Result was inconclusive as transfection efficiency was poor for that particular experiment, if I recall correctly, but I do recall both colours were FACS compatible and flow was mentioned was an application for those reagents. Guy Next generation therapeutic <http://www.ablynx.com/> antibodies Guy Hermans, PhD Senior Scientist Ablynx NV Technologiepark 4 B-9052 Zwijnaarde Belgium guy.hermans@ablynx.com tel: fax: mobile: +32 (0)9 261 06 57 +32 (0)9 261 06 27 +32 (0)486 788 551 <https://www.plaxo.com/add_me?u=30065269879&v0=994426&k0=2009290972> Add me to your address book... <http://www.plaxo.com/signature> Want a signature like this? -----Original Message----- From: Massicotte Éric [mailto:Eric.Massicotte@ircm.qc.ca] Sent: Thursday, October 26, 2006 8:56 PM To: cyto-inbox Subject: beta-gal reagent for flow Hi Flower's community, I was wondering if somebody used a different reagent than FDG for beta-gal tracing in flow cytometry. I found another substrate called DDAO galactoside and since there is no datasheet available on invitrogen's websitefor that product, I have no idea if it's possible to use it for flow cytometry. Thank you for your help!!! _____ Eric Massicotte Responsable, Service de cytométrie en flux Institut de Recherches Cliniques de Montréal (IRCM) 110 ave. des Pins Ouest Montréal, Québec Canada, H2W 1R7 Téléphone: (514) 987-5724 ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. "NANOBODY" and "NANOCLONE" are registered trademarks of Ablynx N.V. -----------------------------------------------------------------------
