Dear Fellow Cytometrists, I have an investigator that is looking for a way to definitively mark osteoprogenitor cells by flow. We have come up with CD40 and CD99 but are wondering if there are other established methods that people are using currently. Most of the literature is about labeling bone marrow mesenchymal cells, which are theoretically the same as osteoprogenitors, and we are unsure as to how we can further purify as what we see labeled as osteoprogenitors. We simply want to count the positively stained cells. Any thoughts or insights are appreciated! Cheers, Phillip Phillip Eckels Flow Cytometry Core Manager Denver Health Medical Center Denver, CO 80204 303-436-5629 ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE - This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender by telephone or return e-mail and delete the original transmission and its attachments without reading or saving in any manner. Thank you. ==============================================================================Received on Wed Oct 18 12:44:14 2006
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