Dear Robin We routinely sort cells directly on microscope slides for subsequent FISH analysis. We use positively charged superfrost slides and never encountered problems. Poly-L-lysine is worked also in our hands. Best regards Karen Karen Hensen, PhD Laboratory of Experimental Hematology Virga Jesse Hospital Stadsomvaart 11 3500 Hasselt Belgium Phone +32-11-309700 Fax +32-11-309750 --- Oorspronkelijk bericht --- Poly-l-lysine? It is what we use to keep not very sticky cells “glued” onto the plastic ware for further work. David ================================= David L. Haviland, Ph.D. Assistant Professor, Immunology Institute of Molecular Medicine, R637I Univ. of Texas, Houston - HSC 1825 Pressler Houston, TX 77030 713-500-2413 - Voice ================================= From: Stingley, Robin L [mailto:StingleyRobinL@uams.edu] Sent: Tuesday, October 03, 2006 1:39 PM To: cyto-inbox Subject: Sorting onto slides Hello all, I vaguely remember during training for the FACSAria that we should coat slides with something to help the cells to stick when sorting directly to slides. Can anyone tell me what it was? Thanks! Robin Robin Stingley, Ph.D. Flow Cytometry Core Facility Department of Microbiology and Immunology University of Arkansas for Medical Sciences Slot 511, Rm B504 4301 West Markham Street Little Rock, Arkansas 72205 Phone: 501-686-6927 E-mail: StingleyRobinL@uams.edu http://www.uams.edu/flowcytometry/ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. >Received on Thu Oct 12 14:18:00 2006
This archive was generated by hypermail 2.1.8 : Sat Oct 14 2006 - 03:12:04 EDT
