RE: Rhodamine123 vs. Hoechst 33342 for side population detection

I would suggest looking at the Invitrogen violet Vybrant dyes, I am in the process of
comparing Aria UV Hoechst to Aria Violet Vybrant , so far I have run 2 stains and the
results look very promising 


Ian Dimmick
Flow Cytometry Core Facility Manager
Institute of Human Genetics
Bioscience Centre
Central Parkway
Newcastle Upon Tyne
NE1 3BZ
UK

Ian.Dimmick@ncl.ac.uk
Tel	0044 191 2418831/ 8825
Fax    0044 1912418666
(mob) 0044 7970344823



-----Original Message-----
From: Albert Tai [mailto:acktai@exelixis.com]
Sent: Fri 06-Oct-06 17:20
To: cyto-inbox
Subject: Rhodamine123 vs. Hoechst 33342 for side population detection
Hi flow cytometer users,


I am trying to identify/sort stem cell side population (SP) using
Hoechst 33342 (based on Goodell et al.) but, unfortunately, our FACS
Aria does not equip with an UV laser.  The excitation of the Hoechst dye
by violet laser may not be optimal.  I am considering of using Rhodamine
123 for my staining and I am wondering if anyone has any success using
it for side population detection.  A point in direction for a working
protocol would be much appreciated.


Thanks you in advance for your time and assistance.


Albert

Exelixis, Inc.



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