IPEC-J2 CELLS

Dear fellow flow-ers:

I am trying to sort some cells for a client and am having real 
trouble.  The cells are porcine intestinal epithelial non-transformed 
primary cell line isolated from the jejunum. They are similar to 
CACO-2 cells.  They are 30-40 um in size.  I am trying to sort them 
on a MoFLo sorter using a 150um tip.  They appear to be especially 
susceptible to destruction and rupture.  We have tried fixing them 
but they still seem to rupture and splouge(their word for spilling 
all the cell contents into the solution).   I was under the 
impression that once fixed, the cells should stay stable and not 
rupture.

Any help would be greatly appreciated.

Thanks as always.

Janet Dow


-- 
Janet Dow
Laboratory Research Specialist and Manager
Flow Cytometry and Cell Sorting  Facility
North Carolina State College of Veterinary Medicine
4700 Hillsborough Street
Room C-309
Raleigh, NC 27606
(919)513-6443